Coding

Part:BBa_K363011:Design

Designed by: Justin Porter   Group: iGEM10_Johns_Hopkins   (2010-10-26)

Cna1, catalytic subunit of calcium sensitive protein phosphatase calcineurin from S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 760
    Illegal BsaI.rc site found at 1303


Design Notes

his gene has been codon optimized for S. cerevisiae and has had restriction enzymes removed such that it is compatible with all five standards.


Source

This part is encoded on the twelfth chromosome of S. cerevisiae.

References

  • Cyert MS. Calcineurin signaling in Saccharomyces cerevisiae: how yeast go crazy in response to stress. Biochem Biophys Res Commun (2003)